LifeGel Digestion Kit


€99.00
Tax excluded

The LifeGel Digestion Kit allows the quick and gentle release of single cells grown on LifeGel while preserving the highest-possible yield. The kit (4 x 4 mL) is ready to use and sufficient for digestion of a 48-well plate.

 

Product is intended for research use only, not for diagnostic or therapeutic purposes.



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LifeGel Digestion Kit

LifeGel Digestion Kit

The LifeGel Digestion Kit allows the quick and gentle release of single cells grown on LifeGel while preserving the highest-possible yield. The kit (4 x 4 mL) is ready to use and sufficient for digestion of a 48-well plate.

 

Product is intended for research use only, not for diagnostic or therapeutic purposes.

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Digestion Kit (digestion of the hydrogel)

  • The main task of 3D culturing is to prepare cell cultures for further biochemical, molecular or imaging analyses. In standard 2D culturing, trypsin is the most commonly used enzyme for the recovery of cultured cells – the enzyme cleans the surface proteins, which then allows the cells to become detached from the substrate and go to the suspension.
  • The Digestion Kit is a set of enzymes used to release cells cultured on LifeGel. Carefully selected groups of enzymes digest the hydrogel, as well as the protein combinations between the cells, which enables single cells to be recovered. In contrast to classic trypsin, these enzymes do not digest the membrane proteins necessary for the proper functioning of the cell, so the kit is therefore safe and gentle on the cells. The released cells can then be used in a number of further experiments such as gene or protein expression analysis, for example, or they can be replanted again.
  • The Digestion Kit contains four 4 mL vials (a total of 16 mL of enzymes) filled with the appropriate enzymes that when added to the LifeGel (in a 1:1 ratio) recover cells for further experiments. As a standard, 16 mL of enzymes are sufficient to digest one culture plate of LifeGel.

 

Specification

Form

Liquid

Color

Light yellow

Content

4 vials x 4 mL of Digestion Kit

Leaflet

Storage

Store at -20°C for 6-month stability or at -80°C
for stability unit expiry date. Protect from light.
Avoid repeated freezing and thawing.

 

 

 

 

 

 

 

 

 

Specific References